Enantioselective Biosynthesis of L-Phenyllactic Acid From Phenylpyruvic Acid In Vitro by L-Lactate Dehydrogenase Coupling With Glucose Dehydrogenase.

ABSTRACT: As a valuable versatile building block, L-phenyllactic acid (L-PLA) has numerous applications in the fields of agriculture, pharmaceuticals, and biodegradable plastics. However, both normally chemically synthesized and naturally occurring PLA are racemic, and the production titer of L-PLA is not satisfactory. To improve L-PLA production and reduce the high cost of NADH, an in vitro coenzyme regeneration system of NADH was achieved using the glucose dehydrogenase variant LsGDH^D255C and introduced into the L-PLA production process. Here an NADH-dependent L-lactate dehydrogenase-encoding variant gene (L-Lcldh1^Q88A/I229A) was expressed in Pichia pastoris GS115. The specific activity of L-LcLDH1^Q88A/I229A (Pp) was as high as 44

SUBMITTER: Zhang D 

PROVIDER: S-EPMC8894805 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature