Identification of Gyromitra infula: A Rapid and Visual Method Based on Loop-Mediated Isothermal Amplification.

ABSTRACT: With mushroom poisoning emerging as one of the most serious food safety problems worldwide, a rapid identification method of poisonous mushrooms is urgently required to investigate the source of poisoning. Gyromitra infula, a kind of poisonous mushroom, contains gyromitrin toxin, which causes epileptogenic neurotoxicity and hemolytic disease. This study aimed to establish a rapid and visual method of G. infula identification based on loop-mediated isothermal amplification (LAMP). A set of specific LAMP primers was designed, and its specificity in G. infula was confirmed against various mushroom species, including its closely related species and other macrofungi. The sensitivity assay showed that the minimum concentration of genomic DNA detected by LAMP was 1 ng/μl. The

SUBMITTER: Xie X 

PROVIDER: S-EPMC8894891 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature