Macrolide susceptibility and molecular characteristics of Bordetella pertussis.

ABSTRACT:

Objective

To analyse macrolide resistance and molecular characteristics of Bordetella pertussis clinical isolates from western China, and to explore the relationship between macrolide-resistance and genotypes.

Methods

Susceptibilities of B. pertussis clinical isolates to erythromycin, azithromycin and clarithromycin were determined by epsilometer test (E-test). Isolated strains were sequenced to ascertain the presence of the 23S rRNA gene A2047G mutation. Strains were typed using multilocus antigen sequence typing, multilocus variable-number tandem-repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE).

Results

Of 58 B. pertussis strains isolated in this study, 46 were macrolide-resistant and 12 were macrolide sensitive. All macrolide-

SUBMITTER: Zhang J 

PROVIDER: S-EPMC8894965 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature