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Histone H2B Deacylation Selectivity: Exploring Chromatin's Dark Matter with an Engineered Sortase.

ABSTRACT: We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and β-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics.

SUBMITTER: Wang ZA

PROVIDER: S-EPMC8895396 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Histone H2B Deacylation Selectivity: Exploring Chromatin's Dark Matter with an Engineered Sortase.

Wang Zhipeng A ZA Whedon Samuel D SD Wu Mingxuan M Wang Siyu S Brown Edward A EA Anmangandla Ananya A Regan Liam L Lee Kwangwoon K Du Jianfeng J Hong Jun Young JY Fairall Louise L Kay Taylor T Lin Hening H Zhao Yingming Y Schwabe John W R JWR Cole Philip A PA

Journal of the American Chemical Society 20220217 8

We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and β-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics ...[more]

PMID: 35175758

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