Improved urine DNA methylation panel for early bladder cancer detection.

ABSTRACT:

Background

Bladder cancer is one of the most common malignancies but the corresponding diagnostic methods are either invasive or limited in specificity and/or sensitivity. This study aimed to develop a urine-based methylation panel for bladder cancer detection by improving published panels and validate performance of the new panel with clinical samples.

Methods

Related researches were reviewed and 19 potential panels were selected. RRBS was performed on a cohort with 45 samples to reassess these panels and a new panel inherited best markers was developed. The new panel was applied with qMSP platform to 33 samples from the RRBS cohort and the results were compared to those of RRBS. Lastly, another larger cohort with 207 samples was used to validate new panel performance with

SUBMITTER: Fang Q 

PROVIDER: S-EPMC8895640 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature