Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE.

ABSTRACT:

Background

Blue Native polyacrylamide gel electrophoresis (BN PAGE) followed by denaturing PAGE is a widely used, convenient and time efficient method to separate thylakoid complexes and study their composition, abundance, and interactions. Previous analyses unravelled multiple monomeric and dimeric/oligomeric thylakoid complexes but, in certain cases, the separation of complexes was not proper. Particularly, the resolution of super- and megacomplexes, which provides important information on functional interactions, still remained challenging.

Results

Using a detergent mixture of 1% (w/V) n-dodecyl-β-D-maltoside plus 1% (w/V) digitonin for solubilisation and 4.3-8% gel gradients for separation as methodological improvements in BN PAGE, several large photosystem (PS) I contai

SUBMITTER: Sarvari E 

PROVIDER: S-EPMC8895881 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature