The role of the epidermis enhancer element in positive and negative transcriptional regulation of ebony in Drosophila melanogaster.

ABSTRACT: The spatiotemporal regulation of gene expression is essential to ensure robust phenotypic outcomes. Pigmentation patterns in Drosophila are determined by pigments biosynthesized in the developing epidermis and the cis-regulatory elements of the genes involved in this process are well-characterized. Here, we report that the known primary epidermal enhancer is dispensable for the transcriptional activation of ebony (involved in light-colored pigment synthesis) in the developing epidermis of Drosophila melanogaster. The evidence was obtained by introducing an approximately 1 kbp deletion at the primary epidermal enhancer by genome editing. The effect of the primary epidermal enhancer deletion on pigmentation and on the endogenous expression pattern of a mCherry-fused ebony allele was examined

SUBMITTER: Akiyama N 

PROVIDER: S-EPMC8895987 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature