A deterministic code for transcription factor-DNA recognition through computation of binding interfaces.

ABSTRACT: The recognition code between transcription factor (TF) amino acids and DNA bases remains poorly understood. Here, the determinants of TF amino acid-DNA base binding selectivity were identified through the analysis of crystals of TF-DNA complexes. Selective, high-frequency interactions were identified for the vast majority of amino acid side chains ('structural code'). DNA binding specificities were then independently assessed by meta-analysis of random-mutagenesis studies of Zn finger-target DNA sequences. Selective, high-frequency interactions were identified for the majority of mutagenized residues ('mutagenesis code'). The structural code and the mutagenesis code were shown to match to a striking level of accuracy (P = 3.1 × 10^-33), suggesting the identification of fun

SUBMITTER: Trerotola M 

PROVIDER: S-EPMC8896162 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature