Reconstruction and analysis of a large-scale binary Ras-effector signaling network.

ABSTRACT:

Background

Ras is a key cellular signaling hub that controls numerous cell fates via multiple downstream effector pathways. While pathways downstream of effectors such as Raf, PI3K and RalGDS are extensively described in the literature, how other effectors signal downstream of Ras is often still enigmatic.

Methods

A comprehensive and unbiased Ras-effector network was reconstructed downstream of 43 effector proteins (converging onto 12 effector classes) using public pathway and protein-protein interaction (PPI) databases. The output is an oriented graph of pairwise interactions defining a 3-layer signaling network downstream of Ras. The 2290 proteins comprising the network were studied for their implication in signaling crosstalk and feedbacks, their subcellular localizations

SUBMITTER: Catozzi S 

PROVIDER: S-EPMC8896392 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature