Joint actions of diverse transcription factor families establish neuron-type identities and promote enhancer selectivity.

ABSTRACT: To systematically investigate the complexity of neuron specification regulatory networks, we performed an RNA interference (RNAi) screen against all 875 transcription factors (TFs) encoded in Caenorhabditis elegans genome and searched for defects in nine different neuron types of the monoaminergic (MA) superclass and two cholinergic motoneurons. We identified 91 TF candidates to be required for correct generation of these neuron types, of which 28 were confirmed by mutant analysis. We found that correct reporter expression in each individual neuron type requires at least nine different TFs. Individual neuron types do not usually share TFs involved in their specification but share a common pattern of TFs belonging to the five most common TF families: homeodomain (HD), basic helix loo

SUBMITTER: Jimeno-Martin A 

PROVIDER: S-EPMC8896470 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature