RNA Interference Analysis Reveals the Positive Regulatory Role of Ferritin in Testis Development in the Oriental River Prawn, Macrobrachium nipponense.

ABSTRACT: Ferritin plays an essential role in organismic and cellular iron homeostasis in Macrobrachium nipponense. In this study, we aimed to investigate the role of ferritin in the sexual development of male M. nipponense. According to the qPCR analysis of different tissues and developmental stages, ferritin exhibited high expression levels in the testis and androgenic gland, from post-larval developmental stage 5 (PL5) to PL15, indicating that it may be involved in gonad differentiation and development, especially in male sexual development. In situ hybridization and qPCR analysis in various reproductive cycles of the testis indicated that ferritin may play an essential role in spermatogonia development in M. nipponense. RNAi analysis revealed that ferrit

SUBMITTER: Jin S 

PROVIDER: S-EPMC8896479 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature