Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice.

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) were differentiated into a specific mesoderm subset characterized by KDR⁺CD56⁺APLNR⁺ (KNA⁺) expression. KNA⁺ cells had high clonal proliferative potential and specification into endothelial colony-forming cell (ECFCs) phenotype. KNA⁺ cells differentiated into perfused blood vessels when implanted subcutaneously into the flank of nonobese diabetic/severe combined immunodeficient mice and when injected into the vitreous of type 2 diabetic mice (db/db mice). Transcriptomic analysis showed that differentiation of hiPSCs derived from diabetics into KNA⁺ cells was sufficient to change baseline differences in gene expression caused by the diabetic status and reprogram

SUBMITTER: Gil CH 

PROVIDER: S-EPMC8896785 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature