Cross-talk between GABAergic postsynapse and microglia regulate synapse loss after brain ischemia.

ABSTRACT: Microglia interact with neurons to facilitate synapse plasticity; however, signal(s) contributing to microglia activation for synapse elimination in pathology are not fully understood. Here, using in vitro organotypic hippocampal slice cultures and transient middle cerebral artery occlusion (MCAO) in genetically engineered mice in vivo, we report that at 24 hours after ischemia, microglia release brain-derived neurotrophic factor (BDNF) to downregulate glutamatergic and GABAergic synapses within the peri-infarct area. Analysis of the cornu ammonis 1 (CA1) in vitro shows that proBDNF and mBDNF downregulate glutamatergic dendritic spines and gephyrin scaffold stability through p75 neurotrophin receptor (p75^NTR) and tropomyosin receptor kinase B (TrkB) receptors, respectively. Afte

SUBMITTER: Cramer T 

PROVIDER: S-EPMC8896802 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature