A glass bead semi-hydroponic system for intact maize root exudate analysis and phenotyping.

ABSTRACT:

Background

Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage.

Results

This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl₂, and to measure root phenotype data using standard methods and the Digital imaging of root traits (DIRT) soft

SUBMITTER: Lopez-Guerrero MG 

PROVIDER: S-EPMC8897885 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature