Study on the Mechanism of MicroRNA551b-5p in Severe Acute Pancreatitis Capillary Leakage Syndrome.

ABSTRACT:

Objective

This study focused on investigating the effects of microRNA551b-5p (miR-551b-5p) on severe acute pancreatitis.

Methods

Initially, quantitative real-time polymerase chain reaction (qPCR) is employed to determine the expression of miR-551b-5p in differentiated human umbilical vein endothelial cells (HUVECs). Further, the effects of aberrantly expressed miR-551b-5p in HUVECs Transwell assay. The expressions of proteins associated with severe acute pancreatitis capillary leakage syndrome are determined by Western blot, FITC-phalloidin, and immunofluorescence stainings. Finally, the correlative factor and the target genes of miR-551b-5p, as well as their contributions, are assessed.

Results

We observed that overexpression of miR-551b-5p distinctly promoted the ex

SUBMITTER: Yao Q 

PROVIDER: S-EPMC8898106 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature