Glutamate in primary afferents is required for itch transmission.

ABSTRACT: Whether glutamate or itch-selective neurotransmitters are used to confer itch specificity is still under debate. We focused on an itch-selective population of primary afferents expressing MRGPRA3, which highly expresses Vglut2 and the neuropeptide neuromedin B (Nmb), to investigate this question. Optogenetic stimulation of MRGPRA3⁺ afferents triggers scratching and other itch-related avoidance behaviors. Using a combination of optogenetics, spinal cord slice recordings, Vglut2 conditional knockout mice, and behavior assays, we showed that glutamate is essential for MRGPRA3⁺ afferents to transmit itch. We further demonstrated that MRGPRA3⁺ afferents form monosynaptic connections with both NMBR⁺ and NMBR^- neurons and that NMB and glutama

SUBMITTER: Cui L 

PROVIDER: S-EPMC8898340 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature