Accurate prediction of metagenome-assembled genome completeness by MAGISTA, a random forest model built on alignment-free intra-bin statistics.

ABSTRACT:

Background

Although the total number of microbial taxa on Earth is under debate, it is clear that only a small fraction of these has been cultivated and validly named. Evidently, the inability to culture most bacteria outside of very specific conditions severely limits their characterization and further studies. In the last decade, a major part of the solution to this problem has been the use of metagenome sequencing, whereby the DNA of an entire microbial community is sequenced, followed by the in silico reconstruction of genomes of its novel component species. The large discrepancy between the number of sequenced type strain genomes (around 12,000) and total microbial diversity (10⁶-10¹² species) directs these efforts to de novo assembly and binning. Unfortu

SUBMITTER: Goussarov G 

PROVIDER: S-EPMC8898458 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature