Molecular prevalence and subtyping of Cryptosporidium spp. in fecal samples collected from stray cats in Izmir, Turkey.

ABSTRACT:

Background

Cryptosporidium spp. are obligate intracellular apicomplexan parasites transmitted to humans and other animals by contaminated water, food, or direct contact. They mainly cause gastrointestinal symptoms, although subclinical infections are also common. Cats are primarily infected by host-adapted Cryptosporidium felis while C. parvum and C. muris have also been detected in some cases. In this study, the molecular prevalence of Cryptosporidium spp. was investigated by screening 399 fecal samples collected from stray cats using nested PCR targeting the 18S rRNA gene for the first time in Turkey. Additionally, Cryptosporidium PCR-positive samples were genotyped by nested PCR- restriction fragment length polymorphism (RFLP), and subsequently, amplicons of 18S SSU rRNA were se

SUBMITTER: Koseoglu AE 

PROVIDER: S-EPMC8898748 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature