Testis-enriched Asb12 is not required for spermatogenesis and fertility in mice.

ABSTRACT:

Background

Members of the ankyrin repeat and SOCS box (Asb) family are expressed abundantly in testes. Some Asb genes/proteins are required for spermatogenesis, but the function of Asb12 during spermatogenesis is not clear. We investigated the physiological role of Asb12 in murine testes.

Methods

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 system was used to generate Asb12-knockout (KO) mice. Histology and immunostaining were done to assess the effects of Asb12 KO on mouse testes and epididymides. Semen quality was analyzed using a computer-assisted sperm analyzer. The terminal deoxynucleotidyl transferase-dUTP nick-end labeling assay was employed to examine testicular apoptosis. Real-time reverse transcripti

SUBMITTER: Zhang R 

PROVIDER: S-EPMC8899140 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature