Loss of NLRP3 increases bacterial cystitis via IRAKM.

ABSTRACT:

Background

We attempted to characterize the molecular mechanisms that underpin urinary tract infections using a mouse model of cystitis induced by bacterial infection in a background of NOD-, LRR- and PYD domains-containing protein (NLRP3) deficiency.

Methods

Male NLRP3 knockout (NLRP3^-/-) and control mice (12 weeks old) were intraurethrally inoculated with 2×10⁸ Escherichia coli (E. coli) and euthanized 1, 3, and 7 days later to assess the degree of bladder infection. Immunohistochemical detection of NLRP3 and interleukin-1 receptor-associated kinase M (IRAKM) was performed. Quantitative PCR analysis was performed to analyze the expression of interleukin (IL)-1β and tumor necrosis factor (TNF)-α.

Results

Bladder infection was obser

SUBMITTER: Sun J 

PROVIDER: S-EPMC8899148 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature