Nuclear magnetic resonance footprint of Wharton Jelly mesenchymal stem cells death mechanisms and distinctive in-cell biophysical properties in vitro.

ABSTRACT: The importance of the biophysical characterization of mesenchymal stem cells (MSCs) was recently pointed out for supporting the development of MSC-based therapies. Among others, tracking MSCs in vivo and a quantitative characterization of their regenerative impact by nuclear magnetic resonance (NMR) demands a full description of MSCs' MR properties. In the work, Wharton Jelly MSCs are characterized in a low magnetic field (LF) in vitro by using different approaches. They encompass various settings: MSCs cultured in a Petri dish and cell suspensions; experiments- 1D-T ₁ , 1D-T ₂ , 1D diffusion, 2D T ₁ -T ₂ and D-T ₂ ; devices- with a bore aperture and single-sided one. Complex NMR analysis with the aid of random walk simulations allows

SUBMITTER: Krzyzak AT 

PROVIDER: S-EPMC8899161 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature