Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey.

ABSTRACT: Fertility preservation for prepubertal cancer patients prior to oncologic treatment is an emerging issue, and non-human primates are considered to constitute suitable models due to the limited availability of human testicular tissues. However, the feasibility of spermatogonial stem cell (SSC) propagation in vitro and autologous testicular germ cell transplantation in vivo requires further exploration in monkeys. Herein, we characterized germ cells in macaque testes at 6 months (M), 18 M and 60 M of age, and effectively isolated the spermatogenic cells (including the spermatogonia) from macaque testes with high purity (over 80%) using combined approaches of STA-PUT separation, Percoll gradients and differential plating. We also generated recipient monkey testes with ablated endogenous sperm

SUBMITTER: Yuan H 

PROVIDER: S-EPMC8899175 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature