Adipose-derived stem cell spheroids are superior to single-cell suspensions to improve fat autograft long-term survival.

ABSTRACT: Autologous fat transplantation is a widely used procedure for surgical reconstruction of tissues. The resorption rate of this transplantation remains high and unpredictable, reinforcing the need of adjuvant treatments that increase the long-term stability of grafts. Adipose-derived stem cells (ASC) introduced as single cells in fat has been shown clinically to reduce the resorption of fat grafts. On the other hand, the formulation of ASC into cell spheroids results in the enhancement of their regenerative potential. In this study, we developed a novel method to produce highly homogeneous ASC spheroids and characterized their features and efficacy on fat transplantation. Spheroids conserved ASC markers and multipotency. A regenerative gene expression profile was maintained, and genes linked

SUBMITTER: El Harane S 

PROVIDER: S-EPMC8899177 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature