The Effect of Topical Anesthetics on 16S Ribosomal Ribonucleic Acid Amplicon Sequencing Results in Ocular Surface Microbiome Research.

ABSTRACT:

Purpose

To clarify the short-term effect of topical anesthetics on 16S ribosomal ribonucleic acid amplicon sequencing results in ocular surface microbiome research.

Methods

Both eyes of 24 eligible volunteers undergoing general anesthesia were sampled. Before sampling, a drop of artificial tears or a drop of topical anesthetic was applied in a randomized way. By using artificial tears as a control, we assured blinding of the executer and took a potential diluting effect into account. Bacterial DNA was extracted using the QIAGEN RNeasy PowerMicrobiome Kit with specific adaptations. Amplified DNA was sequenced with the Illumina MiSeq sequencing platform.

Results

Four sample pairs were excluded due to low yield of bacterial DNA. In the remaining 20 sample pairs, no diffe

SUBMITTER: Delbeke H 

PROVIDER: S-EPMC8899854 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature