The NLRP1 Inflammasome Induces Pyroptosis in Human Corneal Epithelial Cells.

ABSTRACT:

Purpose

Inflammasomes are multiprotein complexes that detect danger-associated signals and trigger an immunostimulatory form of cell death called pyroptosis. NLRP1 is an innate immune receptor that assembles into an inflammasome, but the primary cell types in which NLRP1 is functional have not yet been fully established. Mutations in NLRP1 are associated with diseases of barrier epithelial tissues, including skin lesions and corneal intraepithelial dyskeratosis, suggesting that NLRP1 functions within the eye. Here, we investigated the expression and activity of the NLRP1 inflammasome in primary human corneal epithelial (pHCE) cells.

Methods

The small molecule Val-boroPro (VbP) activates the NLRP1 inflammasome. Proteasome (bortezomib, MG132) and caspase-1 (VX-765, Z-VAD-FMK)

SUBMITTER: Griswold AR 

PROVIDER: S-EPMC8899863 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature