Comparative Genome Analysis Reveals <i>Cis</i>-Regulatory Elements on Gene-Sized Chromosomes of Ciliated Protists.

ABSTRACT: Gene-sized chromosomes are a distinct feature of the macronuclear genome in ciliated protists known as spirotrichs. These nanochromosomes are often only several kilobase pairs long and contain a coding region for a single gene. However, the ways in which transcription is regulated on nanochromosomes is still largely unknown. Here, we generated macronuclear genome assemblies for two species of Pseudokeronopsis ciliates to better understand transcription regulation on gene-sized chromosomes. We searched within the short subtelomeric regions for potential cis-regulatory elements and identified distinct AT-rich sequences conserved in both species, at both the 5' and 3' end of each gene. We further acquired transcriptomic data for these species, which showed the 5' cis-regu

SUBMITTER: Zheng W 

PROVIDER: S-EPMC8899921 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature