Exhibition of antifungal resistance by sterol-auxotrophic strains of Candida glabrata with intact virulence.

ABSTRACT:

Background

Candida glabrata is an emerging fungal pathogen in immune-compromised hosts. Previously undetected C. glabrata isolates were successfully recovered from clinical specimens by adding sterols to the growth medium. The clinical isolates are unable to synthesize ergosterol but can take up exogenous sterols under aerobic conditions.

Objectives

This study characterizes the sterol-auxotrophic C. glabrata strains, examines the mutation(s) in sterol synthesis genes, characterizes the drug susceptibility and evaluates the virulence in a mouse infection model.

Methods

Drug susceptibility of the C. glabrata strains was evaluated in a sterol-supplemented medium. The coding sequences of the sterol synthesis genes were analysed in six sterol-au

SUBMITTER: Nagi M 

PROVIDER: S-EPMC8900151 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature