A direct, sensitive and high-throughput genus and species-specific molecular assay for large-scale malaria screening.

ABSTRACT:

Background

Infectious disease diagnostics often requires sensitive molecular assays that identify at both genus and species levels. For large scale screening, such as malaria screening for elimination, diagnostic assay can be a challenge, as both the throughput and cost of the assay must be considered. The requirement of nucleic acid extraction hampers the throughput of most molecular assays. Co-amplification of multiple species or multiplex identification either can result in missed diagnosis or are too costly for large-scale screening. A genus- and species-specific diagnostic assay with simplified procedure, high sensitivity and throughput is still needed. This study aimed to develop a sensitive and high-throughput approach for large-scale infectious disease screening.

Methods

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8900325 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature