Implementation of wedged-serial protein crystallography at PROXIMA-1.

ABSTRACT: An approach for serial crystallography experiments based on wedged-data collection is described. This is an alternative method for recording in situ X-ray diffraction data on crystalline samples efficiently loaded in an X-ray compatible microfluidic chip. Proper handling of the microfluidic chip places crystalline samples at geometrically known positions with respect to the focused X-ray interaction area for serial data collection of small wedges. The integration of this strategy takes advantage of the greatly modular sample environment available on the endstation, which allows access to both in situ and more classical cryo-crystallography with minimum time loss. The method represents another optional data collection approach that adds up to the already large set of methods made available

SUBMITTER: Chaussavoine I 

PROVIDER: S-EPMC8900848 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature