Application of ATAC-Seq for genome-wide analysis of the chromatin state at single myofiber resolution.

ABSTRACT: Myofibers are the main components of skeletal muscle, which is the largest tissue in the body. Myofibers are highly adaptive and can be altered under different biological and disease conditions. Therefore, transcriptional and epigenetic studies on myofibers are crucial to discover how chromatin alterations occur in the skeletal muscle under different conditions. However, due to the heterogenous nature of skeletal muscle, studying myofibers in isolation proves to be a challenging task. Single-cell sequencing has permitted the study of the epigenome of isolated myonuclei. While this provides sequencing with high dimensionality, the sequencing depth is lacking, which makes comparisons between different biological conditions difficult. Here, we report the first implementation of single myofibe

SUBMITTER: Sahinyan K 

PROVIDER: S-EPMC8901173 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature