Trophoblast Differentiation Affects Crucial Nutritive Functions of Placental Membrane Transporters.

ABSTRACT: Cytotrophoblasts are progenitor cells that proliferate and fuse to form the multinucleated syncytiotrophoblast layer, implicated in placental endocrine and transport functions. While membrane transporters play a critical role in the distribution of nutrients, hormones, and xenobiotics at the maternal-fetal interface, their selectivity to the syncytiotrophoblast layer is poorly characterized. We aimed to evaluate the regulation of placental transporters in response to trophoblast differentiation in vitro. Experiments were carried out in isolated primary human trophoblast cells before and after syncytialization. Gene expression of six molecular markers and thirty membrane transporters was investigated by qPCR analysis. Subsequently, functional expression was evaluated for proteins inv

SUBMITTER: Karahoda R 

PROVIDER: S-EPMC8901483 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature