Circulating tumor cell methylation profiles reveal the classification and evolution of non-small cell lung cancer.

ABSTRACT:

Background

The ability of circulating tumor cells (CTCs) to identify lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) could improve pathological diagnosis and the selection of treatments for non-small cell lung cancer (NSCLC). Previous studies have shown that deoxyribonucleic acid (DNA) methylation exhibits cell and tissue specificity. Thus, we aimed to explore the methylation status of CTCs in LUAD and LUSC and identify the potential biomarkers.

Methods

We first analyzed Infinium 450K methylation profiles obtained from The Cancer Genome Atlas and Gene Expression Omnibus. We then performed whole-genome sequencing of CTCs in tumor and matched normal lung tissues and white blood cells from 6 NSCLC patients.

Results

The bioinformatics analysis revealed

SUBMITTER: Jiang JH 

PROVIDER: S-EPMC8902094 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature