The up-regulation of LRIG1 expression inhibits the proliferation, apoptosis and invasion of glioma cells.

ABSTRACT:

Objective

To illustrate the role of LRIG1 in regulating the Notch signaling pathway and glioma cell proliferation, apoptosis and invasion.

Methods

The glioma cells (U373) were divided into control group, NC group and LRIG1 group. After transfection, the CCK-8 assay, Transwell assay, and Flow cytometry were used to explore the biological function of LRIG1 in glioma cells. At the end, Western blot was used to detect the expression of LRIG1, Notch1, Hes1, Bcl-2, and Bax.

Results

The LRIG1 expression in U373 cells was remarkably lower than that in normal glial cells (P=0.019). The LRIG1 expression in the LRIG1 group was successfully increased when compared with that in the control group (P=0.004). The cell viability of the LRIG1 group was significantly lower than t

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC8902577 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature