Heterologous Expression and Functional Characterization of CAP18 from Oryctolagus cuniculus.

ABSTRACT:

Background

Antimicrobial peptides belong to the innate defence system of creatures. These peptides attach to the bacterial membrane in order to die microorganisms by penetrating them. Hence, biotechnology researchers pay more attention to produce antimicrobial peptides for use in various fields. The studies showed that rabbit tissue with inflammation and skin ulcers would be producing CAP18 peptide, which belongs to the cathelicidin group.

Methods

In this study, the optimized sequence of the cap18 gene was placed into the pPICZAα plasmid after the alpha-factor signal and transformed into Pichia pastoris (X-33 strain). Purification of the recombinant peptide was done based on its histidine tail at C-terminal, and western blotting method was used to demonstrate the puri

SUBMITTER: Nikpoor M 

PROVIDER: S-EPMC8903354 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature