Dual action of the cannabinoid receptor 1 ligand arachidonyl-2'-chloroethylamide on calcitonin gene-related peptide release.

ABSTRACT:

Background

Based on the current understanding of the role of neuropeptide signalling in migraine, we explored the therapeutic potential of a specific cannabinoid agonist. The aim of the present study was to examine the effect of the synthetic endocannabinoid (eCB) analogue, arachidonyl-2'-chloroethylamide (ACEA), on calcitonin gene-related peptide (CGRP) release in the dura and trigeminal ganglion (TG), as cannabinoids are known to activate G_i/o-coupled cannabinoid receptors type 1 (CB1), resulting in neuronal inhibition.

Methods

The experiments were performed using the hemi-skull model and dissected TGs from male Sprague-Dawley rats. CGRP release was induced by either 60 mM K⁺ (for depolarization-induced stimulation) or 100 nM capsaicin (for transient

SUBMITTER: Christiansen IM 

PROVIDER: S-EPMC8903492 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature