Integrative omics reveals subtle molecular perturbations following ischemic conditioning in a porcine kidney transplant model.

ABSTRACT:

Background

Remote Ischemic Conditioning (RIC) has been proposed as a therapeutic intervention to circumvent the ischemia/reperfusion injury (IRI) that is inherent to organ transplantation. Using a porcine kidney transplant model, we aimed to decipher the subclinical molecular effects of a RIC regime, compared to non-RIC controls.

Methods

Kidney pairs (n = 8 + 8) were extracted from brain dead donor pigs and transplanted in juvenile recipient pigs following a period of cold ischemia. One of the two kidney recipients in each pair was subjected to RIC prior to kidney graft reperfusion, while the other served as non-RIC control. We designed an integrative Omics strategy combining transcriptomics, proteomics, and phosphoproteomics to deduce molecular signatures in kidney tissue t

SUBMITTER: O'Brien DP 

PROVIDER: S-EPMC8903695 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature