Proteomics and functional study reveal kallikrein-6 enhances communicating hydrocephalus.

ABSTRACT:

Background

Communicating hydrocephalus (CH) is a common neurological disorder caused by a blockage of cerebrospinal fluid. In this study, we aimed to explore the potential molecular mechanism underlying CH development.

Methods

Quantitative proteomic analysis was performed to screen the differentially expressed proteins (DEPs) between patients with and without CH. A CH rat model was verified by Hoechst staining, and the co-localization of the target protein and neuron was detected using immunofluorescence staining. Loss-of-function experiments were performed to examine the effect of KLK6 on the synapse structure.

Results

A total of 11 DEPs were identified, and kallikrein 6 (KLK6) expression was found to be significantly upregulated in patients with CH compared with tha

SUBMITTER: Yuan L 

PROVIDER: S-EPMC8903716 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature