Dataset Information

The complete chloroplast genome sequence of Trigonotis peduncularis (Boraginaceae).

ABSTRACT: Trigonotis peduncularis (Boraginaceae) is an annual or biannual herb widely distributed in temperate Asia and East Europe. The complete chloroplast genome of T. peduncularis was sequenced by high-throughput technologies and assembled for the first time. The complete chloroplast genome of T. peduncularis was 147,508 bp in length with a GC content of 37.6%, which includes a large single-copy region (80,546 bp), a pair of inverted repeats (24,877 bp), and a small single copy (17,208 bp). GC content of IR regions (43.3%) were higher than LSC (35.5%) and SSC (31.1%) regions. The genome was predicted to encode 130 genes, of which 114 were unique, including 80 protein-coding genes, 30 tRNA genes, and four rRNA genes. Result from phylogenetic analysis showed that T. peduncularis was sister to Plagiobothrys nothofulvus, and the intergeneric relationships among the six genera sampled in Boraginaceae were well resolved and strongly supported.

SUBMITTER: Wu JH

PROVIDER: S-EPMC8903795 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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The complete chloroplast genome sequence of Trigonotis peduncularis (Boraginaceae).

Wu Jian-Hua JH Li Hui-Min HM Lei Jin-Man JM Liang Ze-Rui ZR

Mitochondrial DNA. Part B, Resources 20220306 3

Trigonotis peduncularis (Boraginaceae) is an annual or biannual herb widely distributed in temperate Asia and East Europe. The complete chloroplast genome of T. peduncularis was sequenced by high-throughput technologies and assembled for the first time. The complete chloroplast genome of T. peduncularis was 147,508 bp in length with a GC content of 37.6%, which includes a large single-copy region (80,546 bp), a pair of inverted repeats (24,877 bp), and a small single copy (1 ...[more]

PMID: 35274042

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Project description:Erigeron breviscapus is a famous medicinal plant. However, the limited chloroplast genome information of E. breviscapus, especially for the chloroplast DNA sequence resources, has hindered the study of E. breviscapus chloroplast genome transformation. Here, the complete chloroplast (cp) genome of E. breviscapus was reported. This genome was 152,164bp in length, included 37.2% GC content and was structurally arranged into two 24,699bp inverted repeats (IRs) and two single-copy areas. The sizes of the large single-copy region and the small single-copy region were 84,657 and 18,109bp, respectively. The E. breviscapus cp genome consisted of 127 coding genes, including 83 protein coding genes, 36 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. For those genes, 95 genes were single copy genes and 16 genes were duplicated in two inverted regions with seven tRNAs, four rRNAs, and five protein coding genes. Then, genomic DNA of E. breviscapus was used as a template, and the endogenous 5' and 3' flanking sequences of the trnI gene and trnA gene were selected as homologous recombinant fragments in vector construction and cloned through PCR. The endogenous 5' flanking sequences of the psbA gene and rrn16S gene, the endogenous 3' flanking sequences of the psbA gene, rbcL gene, and rps16 gene and one sequence element from the psbN-psbH chloroplast operon were cloned, and certain chloroplast regulatory elements were identified. Two homologous recombination fragments and all of these elements were constructed into the cloning vector pBluescript SK (+) to yield a series of chloroplast expression vectors, which harbored the reporter gene EGFP and the selectable marker aadA gene. After identification, the chloroplast expression vectors were transformed into Escherichia coli and the function of predicted regulatory elements was confirmed by a spectinomycin resistance test and fluorescence intensity measurement. The results indicated that aadA gene and EGFP gene were efficiently expressed under the regulation of predicted regulatory elements and the chloroplast expression vector had been successfully constructed, thereby providing a solid foundation for establishing subsequent E. breviscapus chloroplast transformation system and genetic improvement of E. breviscapus.

Dataset Information

The complete chloroplast genome sequence of Trigonotis peduncularis (Boraginaceae).

Publications

The complete chloroplast genome sequence of <i>Trigonotis peduncularis</i> (Boraginaceae).

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