Polymicrobial Aggregates in Human Saliva Build the Oral Biofilm.

ABSTRACT: Biofilm community development has been established as a sequential process starting from the attachment of single cells on a surface. However, microorganisms are often found as aggregates in the environment and in biological fluids. Here, we conduct a comprehensive analysis of the native structure and composition of aggregated microbial assemblages in human saliva and investigate their spatiotemporal attachment and biofilm community development. Using multiscale imaging, cell sorting, and computational approaches combined with sequencing analysis, a diverse mixture of aggregates varying in size, structure, and microbial composition, including bacteria associated with host epithelial cells, can be found in saliva in addition to a few single-cell forms. Phylogenetic analysis reveals a mixtur

SUBMITTER: Simon-Soro A 

PROVIDER: S-EPMC8903893 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature