Correlating single-molecule rupture mechanics with cell population adhesion by yeast display.

ABSTRACT: Here, we present a method based on yeast surface display that allows for direct comparison between population-level cell adhesion strength and single-molecule receptor-ligand rupture mechanics. We developed a high-throughput yeast adhesion assay in which yeasts displaying monomeric streptavidin (mSA) or enhanced mutant mSA were adhered to a biotinylated coverglass submerged in fluid. After exposure to shear stress (20-1000 dyn/cm²) by rapid spinning of the coverglass, cells were imaged to quantify the midpoint detachment shear stress for the cell population. We then performed atomic force microscope single-molecule force spectroscopy (SMFS) on purified mSA variants and identified correlations between single-molecule rupture force distributions and cell population adhesion streng

SUBMITTER: Santos MS 

PROVIDER: S-EPMC8904261 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature