Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples.

ABSTRACT: Loop-mediated isothermal amplification is known for its high sensitivity, specificity and tolerance to inhibiting-substances. In this work, we developed a device for performing real-time colorimetric LAMP combining the accuracy of lab-based quantitative analysis with the simplicity of point-of-care testing. The device innovation lies on the use of a plastic tube anchored vertically on a hot surface while the side walls are exposed to a mini camera able to take snapshots of the colour change in real time during LAMP amplification. Competitive features are the rapid analysis (< 30 min), quantification over 9 log-units, crude sample-compatibility (saliva, tissue, swabs), low detection limit (< 5 copies/reaction), smartphone-operation, fast prototyping (3D-printing) and ability to select the d

SUBMITTER: Papadakis G 

PROVIDER: S-EPMC8904468 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature