Discovery of antimicrobials by massively parallelized growth assays (Me^x).

ABSTRACT: The number of newly approved antimicrobial compounds has been steadily decreasing over the past 50 years emphasizing the need for novel antimicrobial substances. Here we present Me^x, a method for the high-throughput discovery of novel antimicrobials, that relies on E. coli self-screening to determine the bioactivity of more than ten thousand naturally occurring peptides. Analysis of thousands of E. coli growth curves using next-generation sequencing enables the identification of more than 1000 previously unknown antimicrobial peptides. Additionally, by incorporating the kinetics of growth inhibition, a first indication of the mode of action is obtained, which has implications for the ultimate usefulness of the peptides in question. The most promising peptides of the screen are c

SUBMITTER: Koch P 

PROVIDER: S-EPMC8904554 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature