BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos.

ABSTRACT:

Background

During mammalian preimplantation development, as the fertilized egg develops and differentiates, three cell lineages become specified: trophectoderm (TE), epiblast, and primitive endoderm (PrE). Through two steps of cell fate decisions, 16-cell blastomeres develop into TE and an inner cell mass (ICM), and thereafter, the latter differentiates into pluripotent epiblast and PrE. Although bromodomain and extra-terminal domain (BET) proteins, such as BRD4, are necessary for the transcriptional activation of genes involved in the maintenance of mouse embryonic stem cells by occupying their enhancers, their roles in the development of mouse preimplantation are unknown.

Results

To evaluate the effect of BET protein deficiency on cell lineage formation, we cultured preimp

SUBMITTER: Tsume-Kajioka M 

PROVIDER: S-EPMC8905768 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature