DNA demethylation affects imprinted gene expression in maize endosperm.

ABSTRACT:

Background

DNA demethylation occurs in many species and is involved in diverse biological processes. However, the occurrence and role of DNA demethylation in maize remain unknown.

Results

We analyze loss-of-function mutants of two major genes encoding DNA demethylases. No significant change in DNA methylation has been detected in these mutants. However, we detect increased DNA methylation levels in the mutants around genes and some transposons. The increase in DNA methylation is accompanied by alteration in gene expression, with a tendency to show downregulation, especially for the genes that are preferentially expressed in endosperm. Imprinted expression of both maternally and paternally expressed genes changes in F₁ hybrid with the mutant as female and the wild-

SUBMITTER: Xu Q 

PROVIDER: S-EPMC8905802 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature