Potential roles of hsa_circ_000839 and hsa_circ_0005986 in breast cancer.

ABSTRACT:

Background

Breast cancer (BC) is one of the leading causes of death among women around the world. Circular RNAs (circRNAs) are a newly discovered group of non-coding RNAs that their roles are being investigated in BC and other cancer types. In this study, we evaluated the association of hsa_circ_0005986 and hsa_circ_000839 in tumor and adjacent normal tissues of BC patients with their clinicopathological characteristics.

Materials and methods

Total RNA was extracted from tumors and adjacent non-tumor tissues by the Trizol isolation reagent, and cDNA was synthesized using First Strand cDNA Synthesis Kit (Thermo Scientific). The expression level of hsa_circ_0005986 and hsa_circ_000839 was quantified using RT-qPCR. Online in silico tools were used for identifying potentially im

SUBMITTER: Firoozi Z 

PROVIDER: S-EPMC8906031 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature