Isolation, Identification, and Genomic Characterization of Chicken Astrovirus Isolates From China.

ABSTRACT: Chicken astrovirus (CAstV) infection can cause diarrhea, nephritis, stunted growth, and "white chickens" condition, resulting in economic losses to the poultry industry. Currently, a few CAstVs were isolated and a few full-length genome sequences of CAstV have been deposited in the GenBank. In the present study, two CAstV isolates (AAstV/Chicken/CHN/2017/NJ01 and AAstV/Chicken/CHN/2018/CZ01) were successfully isolated by using LMH cells, and we molecularly characterized these two CAstV isolates and observed the effect of these two isolates on hatchability using chicken embryo infection experiment. The genetic analysis demonstrated that these two strains had the typical characteristics of avian astroviruses, which were composed of three open reading frames, 5'UTR, and 3'UTR. The full-length

SUBMITTER: Zhao W 

PROVIDER: S-EPMC8906052 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature