The endoplasmic reticulum proteostasis network profoundly shapes the protein sequence space accessible to HIV envelope.

ABSTRACT: The sequence space accessible to evolving proteins can be enhanced by cellular chaperones that assist biophysically defective clients in navigating complex folding landscapes. It is also possible, at least in theory, for proteostasis mechanisms that promote strict quality control to greatly constrain accessible protein sequence space. Unfortunately, most efforts to understand how proteostasis mechanisms influence evolution rely on artificial inhibition or genetic knockdown of specific chaperones. The few experiments that perturb quality control pathways also generally modulate the levels of only individual quality control factors. Here, we use chemical genetic strategies to tune proteostasis networks via natural stress response pathways that regulate the levels of entire suites of chaperon

SUBMITTER: Yoon J 

PROVIDER: S-EPMC8906867 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature