Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation.

ABSTRACT:

Aim

To investigate changes in extracellular matrix (ECM) gene expression in human trabecular meshwork (HTM) cells in response to mechanical fluid flow stimulation.

Methods

HTM cells were grown on a glass plate coated with 0.02% type I collagen (COL) and exposed to shear stress (0, 0.2, 1.0 dyne/cm²) for 12h. Changes in genes related to the ECM were evaluated by real-time reverse transcriptase-polymerase chain reaction. Phosphorylation of Smad2 protein was investigated by Western blotting.

Results

After mechanical stimulation, COL type 4 alpha 2, COL type 6 alpha 1, and fibronectin-1 mRNA were significantly higher than the static control (P<0.05, <0.05, and <0.01, respectively). The metalloproteinase-2 and plasminogen activator inhibitor-1 mRNA were s

SUBMITTER: Yoshida K 

PROVIDER: S-EPMC8907041 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature