Murine models of renal ischemia reperfusion injury: An opportunity for refinement using noninvasive monitoring methods.

ABSTRACT:

Background

Renal ischemia reperfusion injury (R-IRI) can cause acute kidney injury (AKI) and chronic kidney disease (CKD), resulting in significant morbidity and mortality. To understand the underlying mechanisms, reproducible small-animal models of AKI and CKD are needed. We describe how innovative technologies for measuring kidney function noninvasively in small rodents allow successful refinement of the R-IRI models, and offer the unique opportunity to monitor longitudinally in individual animals the transition from AKI to CKD.

Methods

Male BALB/c mice underwent bilateral renal pedicle clamping (AKI) or unilateral renal pedicle clamping with delayed contralateral nephrectomy (CKD) under isoflurane anesthetic. Transdermal GFR monitoring and multispectral optoacoustic tomog

SUBMITTER: Harwood R 

PROVIDER: S-EPMC8907719 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature