Subtypes in pancreatic ductal adenocarcinoma based on niche factor dependency show distinct drug treatment responses.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) is characterized by abundant stroma in which microenvironmental (niche) factors promote PDAC progression. In mouse models, reduction of the stroma increased the proportion of poorly differentiated PDAC with a worse prognosis. Here, we aimed to clarify the effects of stroma on PDAC that may define the PDAC phenotype and induce distinct therapeutic responses.

Methods

The molecular features of PDAC based on differentiation grade were clarified by genome and transcriptome analysis using PDAC organoids (PDOs). We identified the dependency on niche factors that might regulate the differentiation grade. A three-dimensional co-culture model with cancer-associated fibroblasts (CAFs) was generated to determine whether CAFs provide nic

SUBMITTER: Shinkawa T 

PROVIDER: S-EPMC8908673 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature